Anti-EIF4A1 Polyclonal Antibody
Cat: K000388P
Summary:
| 【Product name】：Anti-EIF4A1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：1973 | 【Swiss Prot】：P60842 |
| 【Tested applications】：WB,IP,IF | |
| 【Recommended dilution】：WB 1:1000-5000. IF 1:50-200. IP 0.5μg-4μg. | |
| 【Immunogen】：A synthetic peptide of human EIF4A1 | |
| 【Public Immunogen Range】：1-100aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Verified picture:
Immunofluorescence analysis of MCF7 cells using EIF4A1 antibody diluted at 1:100
Western blot analysis with EIF4A1 antibody diluted at 1:1000;Lane: HepG2 293T C2C12 C6 Mouse brain.
Immunofluorescence analysis of C6 cells using EIF4A1 antibody diluted at 1:30.