Anti-CPT1A Polyclonal Antibody
Cat: K000391P
Summary:
| 【Product name】：Anti-CPT1A Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：1374 | 【Swiss Prot】：P50416 |
| 【Tested applications】：IF,WB,IHC | |
| 【Recommended dilution】：WB 1:1000-5000. IHC 1:50-200. IF 1:50-200. | |
| 【Immunogen】：Recombinant protein of human CPT1A | |
| 【Public Immunogen Range】：520-720aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Verified picture:
Immunofluorescence analysis of MCF-7 cell using CPT1A antibody. Blue: DAPI for nuclear staining.
Western blot analysis with CPT1A antibody diluted at 1:1000;Lane: MCF7 cells.
Immunofluorescence analysis of HeLa cells using CPT1A antibody diluted at 1:100.