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Anti-MCM4 Polyclonal Antibody
Cat: K003557P
Summary:
【Product name】:Anti-MCM4 Polyclonal Antibody 【Source】:Rabbit
【Isotype】:IgG
【Gene ID】:4173 【Swiss Prot】:P33991
【Species reactivity】:Rat,Mouse,Human
【Tested applications】:IHC,WB
【Immunogen】:A synthetic peptide of human MCM4
【Storage】:Store at -20℃, avoid freeze/thaw cycles.
Background:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Verified picture:
Western blot analysis with MCM4 antibody diluted at 1:1000;Lane: Jurkat K-562 Mouse thymus.
Immunohistochemistry of paraffin-embedded B cell lymphoma(hu) using MCM4 antibody at dilution of 1:200 (x40 lens).
Immunohistochemistry of paraffin-embedded rat pancreas with MCM4 antibody diluted at 1:100.