Anti-6×His Polyclonal Antibody
Cat: K007439P
Summary:
| 【Product name】：Anti-6×His Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Tested applications】：WB | |
| 【Recommended dilution】：WB 1:2000-10000. | |
| 【Immunogen】：A synthetic peptide | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
Verified picture:
Western blot analysis with 6��His antibody diluted at 1:5000; Lane 1-5: Recombinant protein