Anti-6×His Polyclonal Antibody - Cy3
Cat: K007439P - Cy3
Summary:
| 【Product name】：Anti-6×His Polyclonal Antibody - Cy3 | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Tested applications】：ICC IF | |
| 【Recommended dilution】：ICC 1:50-200. IF 1:50-200 | |
| 【Immunogen】：A synthetic peptide | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
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