Anti-EGFR Monoclonal Antibody
Cat: K200028M
Summary:
| 【Product name】：Anti-EGFR Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Affinity purification |
| 【Gene ID】：1956 | 【Swiss Prot】：P00533 |
| 【Tested applications】：IF,IP,IHC,WB | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:100-200. IP 1:100-300. IF 1:100-200. | |
| 【Immunogen】：A synthetic peptide coupled to KLH | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades.
Verified picture:
Immunohistochemistry of paraffin-embedded Human esophageal cancer with EGFR antibody diluted at 1:200.
Western blot analysis with EGFR antibody diluted at 1:2000;Lane: HeLa.
Immunofluorescence analysis of Hela cells using EGFR antibody diluted at 1:100.