Anti-RELA Monoclonal Antibody
Cat: K200045M
Summary:
| 【Product name】：Anti-RELA Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Affinity purification |
| 【Gene ID】：5970 | 【Swiss Prot】：Q04206 |
| 【Tested applications】：IP,IF,WB,IHC | |
| 【Recommended dilution】：WB 1:1000-3000. IP 1:100-300. IF 1:100-200. IHC 1:100-300. | |
| 【Immunogen】：recombinant protein | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
Verified picture:
Western blot analysis with RELA antibody diluted at 1:2000;Lane: HeLa,A431,THP-1.
Immunohistochemistry of paraffin-embedded Human lung cancer with RELA antibody diluted at 1:200.
Immunofluorescence analysis of Hela cells using RELA antibody diluted at 1:100.