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Anti-HSP70 Monoclonal Antibody - SAlexa Fluor 488
Cat: K200048M - SAlexa Fluor 488
Summary:
【Product name】:Anti-HSP70 Monoclonal Antibody - SAlexa Fluor 488 【Source】:Mouse 【Source】:Mouse
【Isotype】:IgG1
【Gene ID】:3303/3304 【Swiss Prot】:P0DMV8/P0DMV9
【Species reactivity】:Human,Pig,Rat,Mouse
【Tested applications】:FC ICC IF
【Recommended dilution】:Flow-Cyt 1:50-200. ICC 1:50-200. IF 1:50-200
【Immunogen】:A synthetic peptide coupled to KLH
【Storage】:Store at -20℃, avoid freeze/thaw cycles.
Background:
The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.
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