Anti-EZH1 Polyclonal Antibody
Cat: K107561P
Summary:
| 【Product name】：Anti-EZH1 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：2145 | 【Swiss Prot】：Q92800 |
| 【Tested applications】：WB,IF,IHC,IP | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:100-300. IF 1:50-200. IP 1:50-500. | |
| 【Immunogen】：A synthetic peptide of human EZH1 | |
| 【Public Immunogen Range】：50-100/747aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Verified picture:
Western blot analysis with EZH1 antibody diluted at 1:2000;Lane: HT-29,SW480,HepG2,K562,MCF7,HeLa,293T.
Immunohistochemistry of paraffin-embedded Human renal cancer with EZH1 antibody diluted at 1:200.
Immunofluorescence analysis of A431 cells using XPR1 antibody diluted at 1:100.
Immunoprecipitation analysis of Hela extracts using EZH1 antibody(IP:3-12ug;WB:1:1000).Control IgG: Normal Rabbit IgG.