Anti-MBP-TAG Polyclonal Antibody
Cat: K107769P
Summary:
| 【Product name】：Anti-MBP-TAG Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：66672051/948538 | 【Swiss Prot】：P0AEX9 |
| 【Tested applications】：WB | |
| 【Recommended dilution】：WB 1:1000-1:3000. | |
| 【Immunogen】：Recombinant protein of E.coli MBP-TAG | |
| 【Public Immunogen Range】：1-396aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest.
Verified picture:
Western blot analysis with MBP-TAG antibody diluted at 1:2000;Lane: Recombinant protein of MBP 3ul,Recombinant protein of MBP 5ul.