Anti-GPR31 Polyclonal Antibody
Cat: K107791P
Summary:
| 【Product name】：Anti-GPR31 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：2853 | 【Swiss Prot】：O00270 |
| 【Tested applications】：IHC,WB,IF | |
| 【Recommended dilution】：IHC 1:50-200. IF 1:50-200. | |
| 【Immunogen】：A synthetic peptide of Human GPR31 | |
| 【Public Immunogen Range】：269-319/319aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.
Verified picture:
Immunohistochemistry of paraffin-embedded Human colorectal cancer with GPR31 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human stomach cancer with GPR31 antibody diluted at 1:100.
Immunofluorescence analysis of A431 cells using GPR31 antibody diluted at 1:50.