Anti-PKM Polyclonal Antibody
Cat: K009452P
Summary:
| 【Product name】：Anti-PKM Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5315 | 【Swiss Prot】：P14618 |
| 【Tested applications】：IHC,WB,ELISA,FCM,IF,ICC | |
| 【Recommended dilution】：WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1ug/Test. ICC 1:100. IF 1:100-500. | |
| 【Immunogen】：A synthetic peptide of human PKM | |
| 【Public Immunogen Range】：255-350/531aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Verified picture:
Western blot analysis with PKM2 antibody diluted at 1:300;Lane: Mouse Heart.
Immunohistochemistry of paraffin-embedded Human lung cancer with PKM antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded human lung cancer tissue with PKM2 antibody diluted at 1:200.
Immunofluorescence analysis of human rectal carcinoma using PKM2 antibody diluted at 1:200.