Anti-Histone H3/HIST3H3 Polyclonal Antibody
Cat: K009469P
Summary:
| 【Product name】：Anti-Histone H3/HIST3H3 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 | 【Swiss Prot】：P68431 |
| 【Tested applications】：WB,ELISA,FCM,IF,ICC,IHC | |
| 【Recommended dilution】：WB 1:10000-50000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. ICC 1:100. IF 1:100-500. | |
| 【Immunogen】：A synthetic peptide of human Histone H3/HIST3H3 | |
| 【Public Immunogen Range】：71-136/136aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination.
Verified picture:
Western blot analysis with Histone H3 antibody diluted at 1:50000;Lane: Raw264.7 Hela SH-SY5Y Molt-4 THP-1 293T.
Immunohistochemistry of paraffin-embedded Human cervical carcinoma using Histone H3/HIST3H3 antibody diluted at 1:400
Immunofluorescence analysis of Rat brain tissue using H3/HIST3H3 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded human cervical carcinoma with HIST3H3 antibody diluted at 1:400.
Immunofluorescence analysis of human brain using Histone H3 antibody diluted at 1:500.