Anti-PARP Polyclonal Antibody
Cat: K009545P
Summary:
| 【Product name】：Anti-PARP Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：142 | 【Swiss Prot】：P09874 |
| 【Tested applications】：ELISA,FCM,IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 0.2μg/Test. IF 1:100-500. | |
| 【Immunogen】：A synthetic peptide of human PARP | |
| 【Public Immunogen Range】：201-300/1014aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
Verified picture:
Western blot analysis with PARP1 antibody diluted at 1:300;Lane: Raji.
Immunohistochemistry of paraffin-embedded Mouse intestines with PARP antibody diluted at 1:500
Immunohistochemistry of paraffin-embedded mouse brain tissue with PARP antibody diluted at 1:400.