Anti-CASP9 Polyclonal Antibody
Cat: K009682P
Summary:
| 【Product name】：Anti-CASP9 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：842 | 【Swiss Prot】：P55211 |
| 【Tested applications】：ELISA,FCM,IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg /Test. IF 1:50-200. | |
| 【Immunogen】：A synthetic peptide of human CASP9 | |
| 【Public Immunogen Range】：351-416/416aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Verified picture:
Western blot analysis with caspase-9 p10 antibody diluted at 1:300;Lane: Mouse Brain.
Immunohistochemistry of paraffin-embedded Rat brain with CASP9 antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded rat pancreas with caspase-9 p10 antibody diluted at 1:200.