Anti-PCNA Polyclonal Antibody
Cat: K109134P
Summary:
| 【Product name】：Anti-PCNA Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5111 | 【Swiss Prot】：P12004 |
| 【Tested applications】：WB,IHC | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:50-200. | |
| 【Immunogen】：Recombinant protein of human PCNA | |
| 【Public Immunogen Range】：1-261aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Verified picture:
Immunohistochemistry of paraffin-embedded Human breast cancer with PCNA antibody diluted at 1:100
Western blot analysis with PCNA antibody diluted at 1:2000;Lane:A431,HepG2,HeLa,Raji,293T,MCF7,C2C12,NIH/3T3, Neuro2a, A549,Jurkat,Mouse testis