Anti-AMPKa1/AMPKa2 Polyclonal Antibody
Cat: K009989P
Summary:
| 【Product name】：Anti-AMPKa1/AMPKa2 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5562/5563 | 【Swiss Prot】：Q13131/P54646 |
| 【Tested applications】：IF,WB | |
| 【Recommended dilution】：WB 1:100-500.IF 1:50-200. | |
| 【Immunogen】：Recombinant protein of human AMPKa1/AMPKa2. | |
| 【Public Immunogen Range】：5-280aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
AMPK belongs to the ser/thr protein kinase family. Alpha subunit is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Verified picture:
Western blot analysis with AMPKa1/AMPKa2 antibody diluted at 1:1000;Lane: HeLa 293T Mouse liver Mouse brain C2C12 Rat liver Rat heart C6.
Immunofluorescence analysis of PC-12 cells using AMPKa1/AMPKa2 antibody diluted at 1:100
Immunofluorescence analysis of NIH3T3 cells using AMPKa1/AMPKa2 antibody diluted at 1:100.