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Recombinant human HIF-1 Alpha protein

Cat:P02341

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 362-555aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: HIF-1 Alpha
【Full Name】: Hypoxia-inducible factor 1-alpha
【Uniprot】: Q16665
【Gene ID】: 3091
【Species Reactivity】: Human Mouse Rat (Bovine Zebrafish Chicken)
【Mol Mass】: 21kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: No activity experimental data, to be verified.
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 2-7 days, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A.The enzyme is found in nearly all cells capable of oxidative metablism.This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.

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