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Recombinant human P53 protein

Cat:P02359

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 178-393aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: P53
【Full Name】: tumor protein p53
【Uniprot】: P04637
【Gene ID】: 7157
【Species Reactivity】: Human Mouse Rat (Fish Dog Horse Pig Monkey)
【Mol Mass】: 24kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway.There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase.Mutations in the chromosome 9 copy of this gene cause citrullinemia.Two transcript variants encoding the same protein have been found for this gene.

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