Summary:
Recombinant human DDIT4 protein
Cat：P02397
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-232aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： DDIT4 |
| 【Full Name】： DNA-damage-inducible transcript 4 |
| 【Uniprot】： Q9NX09 |
| 【Gene ID】： 54541 |
| 【Species Reactivity】： Human Mouse Rat (Bovine Gorilla) |
| 【Mol Mass】： 25kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1).Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB.Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage.Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity.Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity).Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity).Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity.Required for normal neuron migration during embryonic brain development.Plays a role in neuronal cell death
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