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Recombinant human PCNA protein

Cat:P02437

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-261aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: PCNA
【Full Name】: proliferating cell nuclear antigen
【Uniprot】: P12004
【Gene ID】: 5111
【Species Reactivity】: Human Mouse Rat (Bovine Zebrafish Fruit fly Chicken Monkey)
【Mol Mass】: 36kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases.These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids.This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of proline to tRNA molecules.Mutations have been found in this gene in some patients with Alpers syndrome.

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