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Recombinant human APRT protein

Cat:P02509

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-180aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: APRT
【Full Name】: adenine phosphoribosyltransferase
【Uniprot】: P07741
【Gene ID】: 353
【Species Reactivity】: Human Mouse Rat (Bovine Fruit fly)
【Mol Mass】: 20kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences.Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity.There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens.This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L.The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance.Stefins have been proposed as prognostic and diagnostic tools for cancer.

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