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Recombinant human CA1 protein

Cat:P02533

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-261aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: CA1
【Full Name】: carbonic anhydrase I
【Uniprot】: P00915
【Gene ID】: 759
【Species Reactivity】: Human Mouse Rat (Bovine Horse Sheep Rabbit Monkey)
【Mol Mass】: 29kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid.They show extensive diversity in tissue distribution and in their subcellular localization.This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8.It encodes a cytosolic protein that is found at the highest level in erythrocytes.Allelic variants of this gene have been described in some populations.Alternative splicing and the use of alternative promoters results in multiple transcript variants.

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