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Product Introduction:
Cytochrome oxidase (CO) is considered to be an intrinsic enzyme of mitochondrial membrane, which has high activity in cells containing a large number of mitochondria (such as cardiac muscle, renal tubular epithelium, gastric parietal cells and liver cells). This enzyme activity is often used as an indicator of intracellular oxidative metabolism, and also as a marker enzyme of mitochondria.
The principle of Cytochrome Oxidase Stain Kit (DAB Method) is that cytochrome oxidase catalyzes the amino oxidation of DAB's side chain, conducts repeated oxidative polymerization and oxidative cyclization to form insoluble brown Phenazine polymer. This enzyme is sensitive to fixative, so must use fresh sections.
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