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Your Position: Home > Dye Reagents > Enzymatic Stain > Acid Phophatase Stain Kit (Kaplow is/Azo Coupling Method)
Acid Phophatase Stain Kit (Kaplow is/Azo Coupling Method)

Storage:Store at -20℃，avoid light，6 months

  G1491Manual
Acid Phophatase Stain Kit (Kaplow is/Azo Coupling Method)
Cat.No：
G1491
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A

Product Introduction:

Acid phosphatase (ACP) is widely distributed in various tissues, mainly in the lysosome, so it is often used as a lysosome marker enzyme. The acid phosphatase outside of lysozyme exists in endoplasmic reticulum and cytoplasm. Acidic phosphatase is different in all kinds of animals. The optimum pH for its activity is 4.5-5.5.
The reaction principle of Acid Phophatase Stain Kit (Kaplow's/Azo Coupling Method) is that phosphoric acid and naphthol are released by hydrolysis of acid phosphatase with AS-BI as substrate at acidic pH. Naphthol was coupled with diazo salts to form colored products, which were localized in the cytoplasm. It usually used for fresh blood smear, cell smear, frozen section and paraffin section.


Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
小鼠肺石蜡/冰冻切片
阳性位点着紫红色，左侧为石蜡切片染色结果，右侧为冰冻切片染色结果，均为甲基绿复染
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