Formalin-EDTA Decalcifying Solution

Product Introduction:

In the process of tissue sectioning, when some tissues contain bone or calcification, the tissue containing calcium should not be directly embedded in paraffin. This is because the density between calcium and paraffin is different, it is difficult to cut a complete section. It is better to fix the calcium containing tissue before decalcification or both operate at the same time. Then continue next operations such as dehydration, transparency, wax immersion, embedding and slicing.

Formalin-EDTA Decalcifying Solution is mainly composed of formalin, EDTA and so on. The pH value is 7.2-7.4. Compared with the conventional EDTA decalcification solution, the solution has less damage to the tissue structure after decalcification, but the decalcification speed is slower. The advantages of this method are: ① the result of staining is good after EDTA decalcification; ② the damage to tissue structure is smaller than that of conventional EDTA decalcification solution. The disadvantages are: ① the speed of decalcification is very slow, which is not suitable for the use of conventional specimen decalcification; ② the tissue will be slightly hard after decalcification; ③ it is not suitable to determine the end point of decalcification by chemical method.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.