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Product Introduction:
Different amino acids have side chain groups with different chemical properties. Some have basic side chains, some have acid side chains, and the resulting proteins have different numbers of basic and acid groups. These groups will make proteins with different net charges in different pH solutions, and the whole protein molecule has more positive charges, that is, basic protein (isoelectric point tends to be alkaline); The whole protein has more negative charge, that is acid protein (isoelectric point tends to be acid).
The principle of Acid Fast Green Kit is that acid protein combine with acid dye containing positive charge to show color. The most abundant acid protein in cells mainly exists in cytoplasm and nucleolus, so after dyeing, the small part of cytoplasm and nucleolus is dyed green. The staining solution is only used in scientific research field, and is not suitable for clinical diagnosis or other purposes.
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