Annealing Buffer for DNA Oligos, also known as DNA oligonucleotide annealing buffer, can be used not only for the annealing of conventional DNA oligos, Moreover, it is especially suitable for the annealing of DNA Oligo, which is difficult to anneal for the construction of siRNA plasmids. DNA annealing buffer (5×) can effectively avoid the hairpin structure of DNA Oligo itself, and the two Oligo can be directly linked to the purified plasmid after annealing. Usually, a large number of positive clones can be obtained after transformation.
It is very simple to use this reagent, just mix the DNA Oligo to be annealed with the DNA annealing buffer (5×) in a certain proportion, and place it on the PCR machine for about 60 minutes.
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