Superoxide Dismutase from Bovine Erythrocytes Lyophilized Powder

Product introduction

SOD from bovine erythrocytes was the first SOD to be found in mammalian tissues. Before its

enzymatic activity was discovered the protein was known as haemocuprein or erythrocuprein.

Superoxide Dismutase (SOD) catalyzes the conversion of superoxide radicals into hydrogen peroxide

and molecular oxygen.

2 O2–+ 2 H+ → O2+ H2O

SOD from bovine erythrocytes is a homodimeric non-covalently bound protein with two 16.3kDa

subunits of 151 amino acids. Each monomer has one intrachain disulfide and one free sulfhydryl, one Cu2+

atom and one Zn2+ atom.

There are three forms of SOD differentiated by the metal ions in the active site. These are Cu2+/Zn2+

, Mn2+, and Fe2+ SOD. In vertebrate organisms Cu/Zn-SOD is found in the cytoplasm and the mitochondrial

intermembrane space, while Mn-SOD is found in the mitochondrial matrix space. Fe-SOD is found in prokaryotes and some higher plants.

Scope of application

It catalyzes the dismutation of superoxide radicals to form hydrogen peroxide and oxygen molecules. It plays an important role in cellular defense against the toxic effects of oxygen free radicals.

Usage method

SOD is soluble in water (20 mg/ml) yielding a colorless to blue-green solution. SOD is also soluble in aqueous buffers such as 0.1 M potassium phosphate, pH 7.5.

Isoelectric point

4.95

Optimum pH

7.8

pH Stability

7.6–10.5

Optimum temperature

25 ℃

Inhibitors

cyanide, OH–

(competitive), hydrogen peroxide

Matters needing attention

For Laboratory Use Only. Not for drug, household or other uses.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.