Trypsin 1:250
Cat.No：T8150 Solarbio
CAS：9002-07-07
Appearance：White Powder
Storage：Store at 2-8℃，2 years
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
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Pipette & Dispenser & Pipette Dispensing Instrument
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Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Trypsin 1:250 |
| CAS | 9002-07-07 |
| Storage | Store at 2-8℃，2 years |
| Note | NFU/mg, Full name:National Formulary Unit, specifically expressed the enzyme activity potency, and the commonly used potency unit U/mg, IU/mg, similar meaning, just different detection activity method. |
| Appearance | White Powder |
| Grade | Tissue Culture Grade |
| Source | Porcine pancreas |
| Enzyme Activity/Titer | 250.N.F.U/mg。 |
| Solubility | 0.25% PBS solution |
| Unit | Bottle |
| Specification | 10g 25g 100g |
Product introduction
In the in vitro culture of histiocytes, in the primary cell culture of histiocytes, in the digestion and dispersion of adherent growth cells and in the passage cell culture, the digestion and dispersion of adherent growth cells should be used. The commonly used digests are trypsin, EDTA, etc. Their main function is to hydrolyse proteins (such as extracellular matrix) between cells, disperse tissues or adherent cells into single cells, and make cell suspension for further experiments.
Matters needing attention
1. Due to the different properties of tissues or cells, the experimenter should determine the optimal digestion time according to the specific situation. The time of digesting cells should not be too long, otherwise it will affect the cell adhesion and growth.
2. This product does not contain bacteriostatic agents, in the use of the process should pay special attention to aseptic operation, to avoid the digestion of microbial contamination;
3. It is not suitable for long-term storage at 4℃, avoid repeated freezing and thawing, and it is recommended to separate and freeze when used in small amounts.
4. For your safety and health, please wear a lab coat and wear disposable gloves to operate.
In addition, the company has prepared trypsin digestion solution (Cat: T1300 T1320 T1350), which can be directly used for cell digestion.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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您好，前段时间我们公司采购了索莱宝CAS：9002-07-7的胰蛋白酶，有关酶活250.N.F.U/mg，我有几个疑问想请教下，酶活的国际单位是U/ml，这个酶活我可以理解成是250U/mg嘛？其中N.F.是代表什么含义？与国际单位U/ml的换算是怎样的？ 2022-09-17 11:24:25
AdministratorNFU的意思National Formulary Units。和U的意思一样，是不同的说法。
你好我想买个胰蛋白酶含3500U的应该买哪个货号的产品？官网货号T8159的胰蛋白酶1：250是什么意思？ 2022-07-14 17:19:43
Administrator胰蛋白酶活性≥3800u/mg的货号为T8152，胰蛋白酶1：250中1：250是指酶活定义