rTEV Protease
Cat.No：P2060 Solarbio
Appearance：Colourless transparent liquid
Storage：Store at -80℃，transport with dry ice.
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SAlexa Fluor-Conjugated
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Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
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Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | rTEV Protease |
| Storage | Store at -80℃，transport with dry ice. |
| Appearance | Colourless transparent liquid |
| Enzyme Activity/Titer | 5U/ul |
| Unit | Piece |
| Specification | 1000U |
Product introduction
he rTEV protease (Recombination type) is a genetically engineered recombinant protease that specifically recognizes the Glu-Asn-Leu-Tyr-Phe-Gln-Gly seven amino acid sequence. Compared to other proteases such as gut kinase U(EK), Thrombin, FactorXa, and SUMO, rTEV protease exhibits dual characteristics of high activity and specificity. Therefore, it is highly effective in removing fusion tags after expression of fusion proteins.The enzyme was purified by 6×His labeling (containing histamine label), with a purity of 99%. After the shear reaction, the enzyme can be removed by His labeling purification resin Ni-NTA Resin (Cat.No. P2010) to achieve the purpose of purification of the target protein.
Optimum pH
8
Inhibitors
1) PMSF and AEBSF (1 mM), TLCK (1 mM), Bestatin (1 mg/ml), Pestatin A (1 mM), EDTA (1 mM) and E-64 (3 mg/ml), the above protease inhibitors had no effect on rTEV activity; 2) Zn ion (>5 mM) inhibited rTEV activity; 3) Reagents that react with cysteine are also potential inhibitors of rTEV.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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