The TRIzol? Max? Bacterial RNA Isolation Kit contains TRIzol? Reagent and Max Bacterial Enhancement Reagent. Store at 2°C to 8°C.

The TRIzol? reagent is a fully ready-to-use reagent that can be used to extract high quality total RNA or simultaneously extract RNA, DNA, and proteins from a variety of biological samples. This single-phase solution of phenol and guanidine isothiocyanate can be used to extract pure RNA, DNA and proteins from cell and tissue samples of human, animal, plant, yeast or bacterial origin in just one hour.

? Simultaneous extraction of RNA, DNA, and protein from a single sample

? Excellent cracking capability even for difficult sample types

? Optimized formulations and protocols for tissue, cell, serum, viral and bacterial sample types

Obtain reliable purified RNA from multiple sample sizes and sources

TRIzol? reagents are used to treat small amounts of tissues (50-100mg) and cells (A-106) and large amounts of tissues (≥1g) and cells (>107) of human, animal, plant, or bacterial origin, providing excellent performance and accompanied by A sample purification protocol. During sample homogenization, TRIzol? destroys cells, lyses cell components, and effectively inhibits RNA enzyme activity, thereby maintaining RNA integrity. The TRIzol? reagent method is simple and can handle large numbers of samples simultaneously. The whole process can be completed in less than an hour. Total RNA extracted with TRIzol? reagents is not contaminated by proteins and DNA.

Suitable for the extraction of multiple molecular targets

TRIzol? reagents allow you to sequentially precipitate RNA, DNA, and proteins in a single sample. After homogenizing the sample with TRIzol? reagents and adding chloroform, the homogenate can be divided into a transparent upper aqueous layer (containing RNA), a phase interface, and a red lower organic layer (containing DNA and proteins). The RNA is then precipitated from the aqueous layer with isopropyl alcohol. DNA is precipitated from the phase interface/organic layer with ethanol. Protein was precipitated from phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA or protein is washed to remove impurities and re-suspended for downstream application.

For research use only. It shall not be used in the diagnosis or treatment of animals or humans.