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Methotrexate
CAS:1959-05-02
Appearance:Yellow Powder
Storage:Store at -20℃，3 years
Purity:≥99.0%
Product Introduction：
L-02 cells were lysed using radio immunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors, centrifuged and the supernatant collected.100 μg of cellular protein was mixed with 4 times volume of 5X SDS-PAGE loading buffer(with DTT).
Use：
WB;SDS-PAGE
product classification：
SDS-PAGE Protein Sample;Cell Lysate
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
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