Description:

Immunosuppressant. Cyclosporin A, also known as Cyclosporine or Antibiotic S 7481F1, is a commonly used immunosuppressive drug used to stop rejection in organ and bone marrow transplants. Cyclosporin A reduces immune system activity by interfering with T cell activity and growth [1][2]. Cyclosporin A binds to the intracellular receptor cyclophilin of lymphocytes, especially T cells, to form a complex surface that specifically targets calcineurin (phosphatase 2B) activity with an IC50 of 5 nM. Cyclosporin A also inhibits IL-2 production in activated T cells, with an IC50 of 10 nM[3]. Whether cultured FJ17 cells in vitro or FJ17-transfected mouse models, cyclosporin A can inhibit the production of interleukin IL-2 and IL-5, and results show that cyclosporin A can inhibit airway eosinophilic allergic inflammation by inhibiting the synthesis of IL-5 in T cells [4]. cyclosporin A affects mitochondrial function by interfering with the opening of mitochondrial permeability transport pore (MPTP), thereby inhibiting the release of cytochrome c and regulating apoptosis [5].

Use and synthesis method:

Please refer to relevant literature for the specific concentration of

Cyclosporin A, and explore and optimize it according to the experimental conditions (such as experimental purpose, cell type, culture characteristics, etc.).

Application example (for reference only) :

1) To detect the inhibitory activity of Cyclosporin A on calcineurin, Jurkat cells were treated with 100 nM Cyclosporin A for 1h, and then the phosphatase activity was determined. It was found that Cyclosporin A effectively inhibited the Ca2+ dependent phosphatase activity. In addition, Jurkat cells were incubated in the medium containing OKT3 and PMA and different concentrations of Cyclosporin A (0.001-10000nM) for 24h to detect the activity of IL-2 products, and it was found that the IC50 value of Cyclosporin A to inhibit IL-2 was 10nM[3].

2) To test the effect of Cyclosporin A on cytokines in T cells in vitro, FJ17 was cultured with mitomycin-C treated spleen cells for 24h, and Cyclsporin A (1,10,100nM) of different concentrations were added to the culture system. IL-2, IL-4, and IL-5 products were detected and Cyclosporin A was found to inhibit IL-2, IL-4, and IL-5 products in a dose-dependent manner. In addition, 30mg/kg Cyclosporin A was found to significantly inhibit the mRNA expression of IL-2 and IL-5 in vivo [4].

Physical shape:

Odorless and tasteless.

Solubility:

This product is highly soluble in methanol, ethanol or acetonitrile, easily soluble in ethyl acetate, soluble in acetone or ether, and almost insoluble in water.

DMSO dissolved storage solution (100 mg/ml) or ethanol formulated storage solution (200 mg/ml). After packaging, it is recommended to store at -20oC away from light to avoid repeated freezing, and can be stored for at least 6 months.

Note:

1) This product is a chemical reagent and may be harmful to human body. Please do a good job of safety protection and avoid direct contact with skin.

2) This product is used for cell experiments. After dissolution, it must be filtered with disposable needle filter to remove bacteria.

3) This product is only for scientific research purposes and is prohibited for human use.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.