Sometimes the appropriate concentration of antibiotics is added to the cell medium, which can effectively prevent microbial contamination. At present, the most commonly used antibiotic is penicillin-streptomycin mixed solution (100× double antibody). Penicillin-Streptomycin-Gentamicin Solution (100 x) is a triantibody specifically designed for cell culture and can be added directly to cell culture after filtration. The content of penicillin, streptomycin and gentamicin in the mixed solution of penicillin-streptomycin (100× three antibodies) was 10kU/ml, the content of streptomycin was 10mg/ml and the content of gentamicin was 5mg/ml. The solution was prepared with 0.9%NaCl or PBS, and the recommended working concentration of penicillins in cell culture medium was 100U/ml, the working concentration of streptomycin was 0.1mg/ml, and the working concentration of gentamicin was 50μg/ml, that is, it could be used in a 100-fold dilution. A package of 100ml penicillin-streptomycin - gentamicin mixture (100× triple antibody) can prepare 10L cell culture solution.

Penicillin-streptomycin - gentamicin mixed solution (100× triple antibody) can be used in one of the following two ways:

1. Directly add in sterile cell culture medium: add penicillin-streptomycin and gentamicin mixed solution (100× triantibody) at the ratio of 5ml per 500ml cell culture medium, and mix well for use.

2. When preparing cell culture medium, add it, and then filter and remove bacteria: when preparing cell culture medium, add penicillin-streptomycin - gentamicin mixed solution (100× trianti) in the ratio of 10ml for each 1L cell culture medium, and filter and remove bacteria after preparation.

Note: :

1. Minimize the number of repeated freezing and thawing, so as not to reduce efficiency.

2. Pay attention to aseptic operation and try to avoid contamination.

3. For your safety and health, please wear a lab coat and disposable gloves.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.