Products
RIPA Buffer

Storage:Store PMSF at -20℃, lysis buffer at 2-8℃, valid for 1 year.

RIPA Buffer
Cat.No:
R0020
Brand:
Solarbio

Product Description: RIPA tissue/cell lysate is the use of surfactants, etc., to cleave cell membranes (including nuclear membranes). The protein extracted by this reagent is soluble total protein.

Instruction:

If RIPA is found to have precipitation, please let it sit at room temperature for half an hour or in a room temperature water bath to dissolve the precipitation.

According to the amount used, add 10ul PMSF for every 1ml RIPA, so that the final concentration of PMSF is 1mM, mix and set aside (add PMSF now).

1. Sample pretreatment:

a) For adherent cells: Remove the culture medium and wash it again with PBS, normal saline or serum-free culture medium. The lysate was added at the ratio of 150-250 ul lysate per cell volume of the 6-well plate. Blow the gun a few times to make full contact between the lysate and the cells.

b) For suspended cells: Centrifuge the cells and add 150-250ul of lysate according to the number of cells in each well of the 6-well plate. Tap the suspended cells with your fingers to fully lysate them. There should be no obvious cell precipitation after full lysis. If the number of cells is large, it must be divided into 5-10×105 cells/tubes, and then cracked.

c) For tissue samples: Cut the tissue into fine fragments. Add the lysate at a ratio of 150-250ul per 20mg of tissue

(If the cleavage is insufficient, more lysate can be appropriately added, and if a high concentration of protein samples is required, the amount of lysate can be appropriately reduced).

Homogenize with a glass homogenizer until fully cracked.

2. Post-processing:

Centrifuge 10000-14000g of the cracked sample for 3-5 minutes, take the supernatant, and then perform subsequent PAGE, Western and immunoprecipitation operations.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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