English 中文
0 My Cart
+86 010 50973105
Products
Biochemical Reagents
Antibiotics (Biochemical Reagents) Vitamins Carbohydrates and Derivatives Animal & Plant Hormones Surfactants Amino Acids & Peptides & Protein Enzymes & Coenzymes & Inhibitors Bases & Nucleic Acids & Derivatives Acids & Salts & Amines Biochemical Reagents Building Blocks PEG Derivatives
Protein and Immunity
Protein Extraction/Quantification Protein Electrophoresis Other Reagents Immunotechnical Services Recombinant Protein Whole Cell Protein
Small Molecule Compounds
Star Products Inhibitors & Antagonists & Agonists Peptides PROTAC Anti-Infection Plant Hormones Amino Acids & Derivatives Vitamins & Derivatives Carbohydrates & It's Analogues Cosolvent & Solubilizer Dye & Probe Reaction Substrate & Physicochemical Detection
Antibodies
Primary Antibodies Secondary Antibodies Loading Control Antibodies Label Antibodies Antigens Plant Antibodies Viral Antibodies Bacterial Antibodies Small Molecule Antibodies RNA Antibodies
ELISA
Human Rats Mice Other Species Antibody Pairs Self-Prepare Reagents
Biochemical Assay Kits
Micromethod Macromethod HPLC Anti-Reverse Series Oxidation Series Antioxidant Series Coenzyme Ⅰ Series Coenzyme Ⅱ Series Glycolysis Series Glutathione Series Vitamin C Metabolism Series ATP Series Nitrogen Metabolism Series Amino Acid Metabolism series Esterase Series Mitochondrial Respiratory Chain Series Tricarboxylic Acid Cycle Series Protease Series Fatty Acid Metabolism Series Starch Series Sucrose Series Sugar Metabolism Series Ion Series Soil Series Signal Series Transhydrogenase Series Other Series Protein quantification Pectin Series Photosynthesis Series Gluconeogenesis Series Vitamin Series
Standard References
Star Products Control Herbs Standard Solution CP Standards Imported Standards Standard References Derivatization Reagent Ion Pair Reagents Domestic Standard Material UV Spectroscopy Standards
Dye Reagents
Stain Solution Fixation Solution Dye
Cell Biology
Cell Detection Reagents Cell Growth Factor Cell Line Cell Culture Cell Isolation & Digestion Cell Staining & Probe Cell Transfection Limulus Lysate
Molecular Biology
Nucleic Acid Extraction/Purification PCR Nucleic Acid Electrophoresis Recombinant Clone Expression Molecular Hybridization
Chromatography Media
Fillers Prepacked Column
Microbial Culture
Strains Drug Sensi-Disc Culture Medium Others
Instruments & Consumables
Consumables Imported Consumables Pipette Instruments Consignment Products
Universal Buffer
Fermentation Products
Diagnostic Reagents
Magnetic Beads
Purified Magnetic Beads Immunoprecipitation Base Magnetic Beads
Nanomaterials
Graphite/Graphene Carbon Nanomaterials Molecular Sieve Other Nanomaterials Inorganic Nanomaterials Metallic Nanomaterial Graphenoid
Peptides
Substance P & Related Peptides Pituitary Adenylate Cyclase Activating Peptides Bag Cell Peptides Cholecystokinin RGD/RAD Peptides Opioid Peptides Cancer Research Apelin/APLN Viral-Associated Peptides Laminin Peptides Pituitary & Hypothalamic Hormone Melanocyte-Stimulating Hormone/MSH Adrenocorticotropic Hormone/ACTH Growth-Promoting Hormone Neuropeptides Thymopoietin & Thymosin Peptides Endothelin & It's Analogues Protein Kinases & Related Peptides Amyloid Peptides Multiple Sclerosis Peptide Tag Osteoporosis Research Matrix Metalloproteinase Substrate Hormones Calcitonin Antimicrobial Peptides Antioxidant Peptides Insect Neuropeptides Casein Morphine Peptides Enzyme Substrate Peptides Prolactin-Releasing Peptides Enkephalin Brain Natriuretic Peptides Endorphin & Endorphin Thrombin-Related Peptides Others Dynorphin Neurokinin Neuropeptides & Related Peptides Renal Myelin Peptides Growth Hormone & Growth Factor Somatostatin & It's Analogues Leptin Fragment Peptides Bombesin Gastrin Cell-Penetrating Peptides Fibronectin & Extracellular Matrix Peptides Anti-Inflammatory Peptides Natriuretic Peptides A Angiotensinogen Substrates & Inhibitors Angiopoietin & Angiotensin Bradykinin Thrombospondin Hematology Inflammation Research Allatostatins Inhibitor-Related Peptides Cosmetic Peptides
Your Position: Home > Cell Biology > Cell Staining & Probe > Hoechst 33342 Stain solution(1mg/ml)
Hoechst 33342 Stain solution(1mg/ml)

Storage: -20℃, avoid light, valid for 1 year.

  C0031Manual
Hoechst 33342 Stain solution(1mg/ml)
Cat.No：
C0031
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
C0031-10*1ml Inquiry Inquiry Inquiry Inquiry
C0031-1ml Inquiry Inquiry Inquiry Inquiry
Qty：
-
+
Add to cart
Order now
Product Information Related Products Related Paper COA Technical Q&A

 Hoechst 33342，也称bisBenzimide H 33342或HOE 33342，是一种可以穿透细胞膜的蓝色荧光染料，对细胞的毒性较低。Hoechst 33342染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33342也常用于普通的细胞核染色，或常规的DNA染色。Hoechst 33342的最大激发波长为346nm，最大发射波长为460nm；Hoechst 33342和双链DNA结合后，最大激发波长为350nm，最大发射波长为461nm。本Hoechst 33342染色液为即用型，可直接用于固定细胞或组织的细胞核染色，也可直接用于活细胞或组织的细胞核染色。
使用说明：
使用前用生理盐水或PBS将Hoechst 33342染色液稀释100倍，即为工作液。
1. 对于固定的细胞或组织：
a. 对于细胞或组织样品，固定后，适当洗涤去除固定剂。随后如果需要进行免疫荧光染色，则先进行免疫荧光染色，染色完毕后再按后续步骤进行Hoechst 33342染色。如果不需要进行其它染色，则直接进行后续的Hoechst 33342染色。
b. 对于贴壁细胞或组织切片，加入少量Hoechst 33342工作液，覆盖住样品即可；对于悬浮细胞，至少加入待染色样品3倍体积的工作液，混匀。室温放置3-5分钟。
c. 吸除Hoechst 33342染色液，用TBST、PBS或生理盐水洗涤2-3次，每次3-5分钟。
d. 直接在荧光显微镜下观察或封片后荧光显微镜下观察。细胞发生凋亡时，会看到凋亡细胞的细胞核呈致密浓染，或呈碎块状致密浓染。
2. 对于活细胞或组织：
a. 加入适当量Hoechst 33342工作液，必须充分覆盖住待染色的样品，通常对于六孔板一个孔需加入1mL工作液，对于96孔板一个孔需加入100μL工作液。
b. 在适宜于细胞培养的温度下培养20-30分钟。弃染色液，用PBS或培养液洗涤2-3次即可进行荧光检测。
注意事项：
荧光染料都存在淬灭的问题，为减缓荧光淬灭可以使用抗荧光衰减封片剂。建议染色后尽量当天完成检测，活细胞或组织染色后应立即观察。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
SERVICE FOR SCIENTIFIC RESEARCH，RESPONSIBLE FOR LIFE
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist, Beijing, China
Tel: +86 010 50973105
Technical Support Email: service@solarbio.com
Purchaser Email: purchase@solarbio.com
Sales Email: sales@solarbio.com
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.
 ICP Filing Certificate京ICP备12051307号-7