UIP

Product introduction

The SUMO protease, also known as Ulp, is a highly active cysteine protease that recognizes the tertiary structure of the SUMO protein rather than the amino acid sequence, thus efficiently and specifically cutting the SUMO protein from the recombinant fusion protein.SUMO protease can maintain high activity in a wide range of reaction environmental systems. SUMO protease carries a polymerized His label, which facilitates the removal of the protease by affinity chromatography after cutting the fusion protein.

Usage method

1. 10×SUMO Protease Buffer: 500mM Tris-HCl, 10mM DTT, pH 8.0.

2. The ratio of SUMO protease to the target protein requiring enzyme digestion: 1:100.

3. Enzyme cutting system:

Fusion protein 1000 μg

10×SUMO Protease Buffer 20μL

SUMO protease 2μL

ddH2O constant volume to 1000μL

4. Enzyme cutting conditions: It is recommended to cut overnight at 4℃, and users can explore according to the target protein of their research. The following enzyme cutting analysis pictures are for reference.

5. A small number of samples can be used for SDS-PAGE analysis after enzyme digestion. To remove SUMO protease in the system after enzyme digestion, His label can be used to purify the tree

Unit definition

In a 1× SUMO Protease Buffer (50 mM Tris-HCl, 1 mM DTT, pH 8.0) reaction at 30℃ for 1h, the amount of enzyme required to shear more than 85% of the 2 μg substrate was defined as a unit of activity.

Optimum pH

5.5-9.5

Optimum temperature

4-30℃

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.