English
中文
Product Description:
Ethanol cryoprecipitation is the most common method for recovering DNA and RNA from liquid samples. However, ethanol precipitation will lose at least 30% of the nucleic acid in the sample. If the concentration of nucleic acid in the liquid sample is very low, or the DNA<200bp, ethanol precipitation can only recover 50% of the DNA and RNA. Acryl Carrier is a molecular biological grade Acryl polymer solution. Adding 5-10μL of ACRyl carrier during ethanol precipitation can significantly improve the yield of nucleic acid precipitation, and the recovery rate of trace DNA can reach 98-100%, while selectively removing short primer fragments and dNTP. This product has no nucleic acid contamination, no DNase and RNase activity, and does not affect subsequent experiments such as enzyme digestion, ligation, transcription, PCR, transformation transfection, nor does it affect nucleic acid electrophoresis and DNA-protein interaction. Acryl Carrier has become the most commonly used nucleic acid precipitation aid.
Product Application:
(1) Yield of DNA or RNA precipitation. For example, increase plasmid yield, improve RNA extraction rate, etc
(2) Trace DNA or RNA recovery.
(3) Precipitate recovery labeled probe to remove unlabeled dNTP.
How to use:
(1) Add 5-20μL Acryl Carrier to 1 ml DNA or RNA solution to continue the selected nucleic acid precipitation procedure.
(2) Add 5-20μL Acryl Carrier to 1mL TRIpure extraction reagent to continue the RNA extraction operation.
(3) Add 5-20μL Acryl Carrier to 1mL plasmid extraction solution to continue the plasmid extraction operation.
Methods used to improve the efficiency of DNA or RNA precipitation recovery
◆ Add 4-8μL Acryl Carrier to RNA or DNA solution and mix upside down.
◆ Use standard ethanol precipitation to precipitate RNA or DNA. Add 3M PH5.2 sodium acetate solution (RNA-free solution should be used when precipitating RNA) to the final concentration of 0.3M (about 1/10 volume), then add 2 times the volume of anhydrous ethanol, mix and place at room temperature or refrigerator for 10-30min, centrifuge for 10min at 12000 RPM, discard the supernatant, and rinse with 70% ethanol. Remove the supernatant, allow to dry and precipitate, and re-dissolve the precipitate in an appropriate amount of DEPC treated water or other buffer such as TE.
Methods used to increase the yield of DNA or RNA
◆ Add 4-8μL Acryl Carrier for each milliliter of total RNA extraction reagent TRIpure (TRIzol) or DNA extraction reagent DNAzol, then proceed with the following steps according to the instructions for these products.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
