TE buffers are Tris+EDTA buffers, which we generally use as dissolvers or preservatives, primarily to regulate PH. TAE is an electrophoretic buffer used primarily for the electrophoresis of DNA molecules.

TE composition concentration: 10mM Tris-HCl 1mM EDTA PH=8.0

Dosage: 500mL

Preparation method: Measure the following solution into a 500mL beaker 1M Tris-HCl Buffer PH=8.0 5mL 0.5M EDTA PH=8.0 1mL Add about 400mL ddH2O into the beaker and mix evenly; After the solution is fixed to 500mL; Store at room temperature.

TAE is the most widely used buffer system. Its characteristics are that the superhelix is more consistent with the actual relative molecular mass during electrophoresis (the relative molecular mass measured during electrophoresis in TBE will be greater than the actual molecular mass), and the mobility of double-stranded linear DNA in it is about 10% faster than the other two buffers, and TAE buffer will achieve better separation effect when electrophoresis fragments larger than 13kb. The recovery of DNA fragments is also easy to electrophoresis with the TAE buffer system. The disadvantage of TAE is that the buffer capacity is small, and long-term electrophoresis (such as overnight) is not optional unless there is a circulation device to exchange the buffer at the two poles. Preparation method of 50×TAE Buffer: 1. Weigh Tris 242g, Na2EDTA.2H2O 37.2g in 1L beaker; 2. 2. Add about 800mL deionized water to the beaker and stir well; 3. Add 57.1mL of glacial acetic acid and dissolve thoroughly; 4. Add deionized water to 1L and store at room temperature.

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