5X Coomassie Brilliant Blue G250(protein quantitative analysis)
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Cat.No：PC0015 Solarbio
Storage：Store at 2-8℃
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> Protein and Immunity > Protein Extraction/Quantification > Protein Assay Kits > 5X Coomassie Brilliant Blue G250(protein quantitative analysis)
Product Information
| Name | 5X Coomassie Brilliant Blue G250(protein quantitative analysis) |
| Storage | Store at 2-8℃ |
| Unit | Bottle |
| Specification | 100ml 500ml |
Product Introduction:
The Coomassie G-250 dye is bound to the protein in an acidic solution so that the position of the dye's maximum absorption peak (Imax) changes from 465nm to 595nm, and the absorbance value A595 measured is proportional to the protein concentration within a certain concentration range. The protein concentration determined by Bradford method is not affected by the chemical substances in most samples. The concentration of merhydryl ethanol in the sample can be as high as 1M, and the concentration of dithithreitol can be as high as 5mM. However, affected by a slightly high concentration of detergent, it is necessary to ensure that the concentration of SDS is less than 0.1%, and that of Triton X-100 is less than 0.1%. The Tween 20, 60 and 80 were below 0.06%. For samples containing detergents, the BCA protein concentration assay kit produced by Solarbio is recommended.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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是用来染SDS-PAGE凝胶吗
2023-05-25 18:56:52
Administrator您好，本产品适用于蛋白定量，凝胶染色的话建议使用P1300，P1305，G4540这几个货号的产品。
请问该产品中考马斯亮蓝的浓度是多少 2023-03-10 17:06:20
Administrator不好意思，浓度不方便告知。