Product Introduction:

NBT is Nitroblue tetrazolium chloride, a dark blue amorphous slightly soluble substance. BCIP, also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate, is catalyzed by Alkaline phosphatase (AP).

BCIP is hydrolyzed to produce a highly reactive product that reacts with NBT to form an insoluble dark blue to bluish-purple precipitate.

Instructions for use:

1. Take 40ul 25×NBT and add it to 1ml 1×AP reaction buffer, mix it well, and then add 40ul 25×BCIP to mix it well, that is, to form a reaction working liquid. This reaction liquid is best used now. Note: Depending on the membrane size, the amounts of 1×AP reaction buffer, 25×BCIP, and 25×NBT can be scaled up equally.

2. Dip the washed PVDF/NC film into the reaction working liquid and gently shake it at room temperature for warming.

3. When the color depth of the protein band meets the requirements (generally about 20-30 minutes), the membrane is immersed in deionized water to stop the reaction.

Note:

BCIP and NBT are suspected carcinogens, please take necessary preventive measures. Wear gloves during operation, avoid contact with skin as much as possible, and rinse thoroughly after use.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.