Ribosome is a kind of ribonucleoprotein particles in cells, which is mainly composed of RNA and proteins. Its only function is to synthesize amino acids into protein polypeptide chains according to the instructions of mRNA, so ribosomes are molecular machines for protein synthesis in cells. The ribosome has no membrane structure and is mainly composed of proteins (40%) and RNA(60%). According to the sedimentation coefficient, ribosomes can be divided into two types, one (70s) exists in bacteria and other prokaryotes, and the other (80s) exists in the cytoplasm of eukaryotic cells. Some of them float in the cells, some gather together. The yeast ribosome extraction kit provides a full set of reagents for extracting total ribosomes from various yeast samples. The extraction process is simple and convenient. This kit can not be used for ribosome extraction of frozen samples. This kit requires ultracentrifugation. If there is no condition of ultracentrifugation, you can choose a kit of other goods and use low-speed centrifugation to extract ribosomes. The ribosome purity of the kit using ultracentrifugation is higher than that of the kit of low speed centrifugation, and the product of the kit of low speed centrifugation takes a long time to extract and needs to be treated overnight. Please choose according to the needs of the reagent. We can provide protein extraction kits for different samples, such as animal cells, tissues, plants, fungi, yeast, microorganisms, liquid, soil and so on. We can provide kits for different applications of downstream experiments, such as Western Blot and bidirectional electrophoresis; protein extraction kits with and without detergent components, and more than 300 protein extraction kits for protein mass spectrometry detection and Pull-down experiments are available. We can also provide extraction kits for different organelles such as nuclei, mitochondria, lysosomes, chloroplasts and exosome of different samples such as animals and plants.
Applicable sample: Yeast.
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