Ribonuclease H

■ RNase H content

Ribonuclease H (20 to 60 U/μl) 600 U

5×Hybrid RNA Degeneration Buffer 300 μl

■ RNase H description

RNase H is an endonuclease that specifically breaks down RNA strands in RNA-DNA hybrids with a molecular weight of 21,000 and an optimal pH of about 8.0. The active factor is Mg2+ or Mn2+.

■ Preservation

-20℃.

■ Origin

Escherichia coli HB101 containing rnh plasmid (pKH11) and regulator plasmid (pNT203)

■ Activity definition

Using poly(rA)·poly(dT) as substrate, the amount of enzyme required to produce 1 nmol of acid soluble substance within 20 minutes at 30℃and pH7.7 was defined as 1 unit of activity (U).

■ Purity

After the reaction of 1.50 U of this enzyme and 1 μg of λ DNA-Hind III at 37℃for 16 h, the electrophoretic bands of DNA did not change.

When 2.50 U of this enzyme and 1 μg of superhelical pBR322 DNA were reacted at 37℃for 1 hour, the electrophoretic bands of DNA did not change.

When 3.50 U of this enzyme and 1 μg of 16S, 23S rRNA reacted at 37℃for 1 hour, the electrophoretic bands of RNA did not change.

■ Use

1. cDNA Cloning was conducted by Okayama-Berg method.

2. Detection of DNA-RNA hybrids.

3. Remove the Poly (A) end of mRNA in the presence of Oligo (dT).

■ Use attention

This enzyme is purified from E. coli recombinant with few impurities and is a product of high purity. Therefore, even the high concentration of products, a large number of use is not a problem.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.