Cetyltrimethylammonium Bromide

Product introduction：

Cetyltrimethylamine bromide: CTAB is a cationic detergent with the property of precipitating nucleic acids and acidic polyglycans from a solution of low ionic strength, in which the protein and neutral polyglycans remain in the solution, in a solution of high ionic strength, forming complexes with proteins and other polyglycans other than most acidic polyglycans, except that nucleic acids cannot be precipitated.

How it Works:

CTAB is a cationic detergent that precipitates nucleic acids and acidic polysaccharides from a solution of low ionic strength, in which the protein and neutral polysaccharide remain in the solution, and in a solution of high ionic strength, can form a complex with the protein and most other polysaccharides other than acidic polysaccharides, but cannot precipitate the nucleic acid, thus separating the nucleic acid.

How to use:

1. Extract DNA formula:

CTAB 4g

NaCl 16.364 g

1M Tris-HCl 20 mL(PH8.0)

After cooling, 0.2-1% 2-mercaptoethanol (400ul) chloroform-isoamyl alcohol (24:1) : first add 96ml chloroform, then add 4ml isoamyl alcohol, shake well.

2. Extract the RNA formula

2% CTAB (W/V)

2% polyvinylpyrrolidone (W/V)

100 mM Tris-HCl (pH8.0,DEPC treated water formulation)

25mM EDTA

Since Tris-HCl reacts with DEPC under high temperature sterilization conditions, the RNA extraction buffer is directly treated with DEPC water.

Use/Application：

It is used to prepare purified DNA from organisms that produce mucopolysaccharides in large quantities, such as plants, and from certain Gram-negative bacteria, including certain strains of E.coli.

Precaution：

It is irritating and harmful if inhaled, ingested or absorbed through the skin. Wear proper gloves and protective glasses when using (or operating in a fume hood). Avoid inhaling its dust.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.