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Appearance:Colourless transparent liquid
Storage:2-8℃, avoid light, valid for 1 year.
This product contains glucose and does not contain calcium chloride, please refer to the instructions for more details.
Product type: Sterile filter ready-to-use liquid medium
Osmotic pressure: 270 ± 15 Acid alkalinity: 7.3 ± 0.2
Description:
As early as the 19th century, researchers explored how to grow animal tissues in vitro, and balanced salt solution was an early solution used in tissue culture. In 1985, Sydney Ringer prepared a salt solution consisting entirely of inorganic salt components to successfully maintain contractility of mammalian myocardial tissue in vitro. Tyrode then prepared a low specificity Tyrode salt solution that was widely used in mammalian cell work, and this medium was widely accepted at the time as a mother solution for diluting other components, such as proteins. Since then, a variety of balanced salt solutions have been developed for tissue and cell culture, and according to their role, the currently commonly used balanced salt solutions are divided into the following categories:1) Provide water and inorganic salts for cell growth and proliferation to meet normal cell metabolism;
2) to provide a suitable acid-base environment for cell growth, the general pH range is between 7.2 and 7.6;
3) Provide amino acids and other nutrients to meet the needs of cell growth, proliferation and metabolism;
4) Add glucose and metabolic intermediates to meet the energy needs of cells;
5) to provide a suitable osmotic pressure environment for cells.
References:
Krebs HA and Henseleit K. Studies on urea formation in the animal organism. Hoppe-Seylers Z.Physiol. Chem. 210:33-66, 1932.
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