Western Blocking Buffer
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Cat.No：SW3010 Solarbio
Storage：Store at 2-8℃，1 month
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Hippo
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Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Western Blocking Buffer |
| Storage | Store at 2-8℃，1 month |
| Unit | Bottle |
| Specification | 100ml 500ml |
Product Introduction:
Western Blot membrane Blocking Buffer is suitable for sealing transfer membranes such as PVDF membrane or NC membrane in Western Blot experiments. After sealing, the non-specific binding of subsequent primary or secondary antibodies to the film can be reduced, the background can be reduced, and the signal-to-noise ratio can be enhanced to achieve the ideal color rendering effect. According to the calculation of 5-10 ml of sealing liquid required for each film sealing, a package of Western sealing liquid can seal 10-20 films.
Usage:
This product is ready-to-use and does not require dilution. After the protein transfer, the transfer film is placed in a container, a sufficient amount of membrane sealing liquid is added to fully cover the film, and the closure is completed by oscillating and sealing at room temperature for half an hour to 1 hour, and then subsequent operations such as primary antibody incubation are carried out.
Note:
It is usually closed at room temperature for 60 minutes. For some antibodies with very high backgrounds, they can be closed overnight at 4 ℃. For your safety and health, please wear a lab coat and disposable gloves.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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