Tris (Hydroxymethyl) Aminomethane

Tris is an organic compound, white to slightly yellow crystalline powder or block, chemical formula C4H11NO3, soluble in ethanol and water, slightly soluble in ethyl acetate, benzene, insoluble in ether, carbon tetrachloride. Solubility at 25℃ (mg/mL) : water 550, ethylene glycol 79.1, methanol 26, absolute ethanol 14.6, 95% ethanol 22.0, N, N-dimethylformamide 14, pentanol 2.6, propanol 2.0, ethyl acetate 0.5, cyclohexane 0.1. The aqueous solution does not absorb carbon dioxide and is corrosive and irritating to copper and aluminum. It is a solvent for nucleic acids and proteins and is widely used in the preparation of buffers in biochemical and molecular biology experiments.

Tris Buffer capacity:

Tris is a weak base with a pKa of 8.1 at 25 ℃. According to buffer theory, the effective buffer range of Tris buffer is between pH7.0 and 9.2. The pH of 0.1mol/l aqueous solution is 10.4, and generally hydrochloric acid is added to adjust the pH value to obtain the buffer with the desired pH value. But at the same time, attention should be paid to the effect of temperature on the pKa of Tris. For Tris buffer, for every 1 ℃ decrease in temperature, the pH increases by about 0.03 units, and for every 10 times dilution of concentration, the pH decreases by 0.03-0.05 units. For precise applications, a carefully calibrated pH meter with a glass/calomel composite electrode is used.

The main applications of Tris are:

1. 1M Tris-HCl 6.8, 1.5M Tris-HCl 8.8 and 5× Tris-glycine electrophoresis buffer were the most commonly used reagents for SDS-PAGE.

2. Adding EDTA to Tris hydrochloric acid buffer to make "TE buffer", TE buffer is used for DNA stabilization and storage. "TAE (Tris/Acetate/EDTA) buffer" was obtained by replacing hydrochloric acid with acetic acid, and "TBE (Tris/Borate/EDTA) buffer" was obtained by replacing boric acid." TAE and TBE buffers are commonly used in nucleic acid electrophoresis experiments.

3. Other applications: Tris was used to grow protein crystals under different pH conditions. The low ionic strength characteristic of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans.

Storage conditions: Dry storage at room temperature.

Precautions: if inhaled, ingested, skin absorption, harmful to the body, use attention to protection.

Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris was used for protein crystal growth at various pH conditions. The low ionic strength characteristic of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer, which forms a buffer system with glycine in the electrophoresis buffer to stabilize the PH in the process of electrophoresis. In addition, Tris is an intermediate in the preparation of surfactants, vulcanization promoters, and some drugs. Tris was also used as a titration standard. As a protein buffer, if the subsequent work requires mass spectrometry, Tris is not suitable, it is best to change to other mass spectrometer can tolerate the buffer.

Often used to configure buffers:

T1010 1.5M Tris-HCL (pH8.8)

T1020 1M Tris-HCL (pH6.8)

T1090 1M Tris-HCL(pH=7.4)

T1130 1M Tris-HCl(pH=7.0)

T1140 1M Tris-HCl(pH=7.5)

T1150 1M Tris-HCl(pH=8.0)

T1160 1M Tris-HCl(pH=9.0)

T1050 5×TBE buffer

T1051 10×TBE buffer

T1060 50×TAE buffer

T1070 5× Tris-glycine electrophoresis buffer

T1510 10x Tris-mops buffer

T1510 10x Tris-mops buffer

T1120 TE buffer, pH=8.0

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

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