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My CartAppearance：Yellow or Green Powder
Molecular Weight：561.93
Storage：Store at -20℃，3 years
Molecular Formula：C27H28N6O·3HCl
CAS：23491-52-3
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| Name | Bis Benzimide Hoechst NO33342 |
| CAS | 23491-52-3 |
| Molecular Formula | C27H28N6O·3HCl |
| Molecular Weight | 561.93 |
| Purity/Grade | ≥98%(TLC) |
| Appearance | Yellow or Green Powder |
| Storage | Store at -20℃，3 years |
| Unit | Piece |
| Specification | 25mg |
中文名称（Chinese Synonym）： 二苯甲亚胺, 三氯化氢 2-(4-乙基苯基)-5-(4-甲基-1-哌嗪基)-2,5-二-1H-苯并咪唑
英文名称（English Synonym） ：2’-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole, trihydrochloride
说明：荧光色素；可与弗伦德红白血病细胞可逆地结合；用于DNA、染色体和核酸的染色；荧光染色法和免疫荧光技术。可用于荧光显微镜或流式细胞仪。
物理性状：溶于水，参考浓度为49.00 - 51.00 mg/ml。
荧光光谱（Fluorescence Spectral）：Hoechst 33342的Ex/Em=346/460; Hoechst33342-核酸的Ex/Em=350/461. 荧光可用氙气灯，泵弧灯或者UV激光激发；可用DAPI滤光器、蓝色GFP滤光器、Alexa Fluor 350滤光器等检测。
Hoechst 33342 是一种可以穿透细胞膜的蓝色荧光染料，它在嵌入双链 DNA 后释放强烈的蓝色荧光，对细胞的毒性较低。Hoechst 33342 常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。也可以用于普通的细胞核染色，或常规的DNA染色。
用于细胞核染色时，推荐的Hoechst 33342工作浓度为0.5-10μg/ml。如需特定浓度或者经浓度优化的产品，可选购Hoechst 33342染液(1mg/ml)（Cat No.C0031）、Hoechst 33342染液(即用型)（Cat No. C0030）。
注意事项:
(1)用于细胞实验，溶解后，须用一次性针头滤器过滤除菌。
(2)为了您的安全和健康，请穿实验服并戴一次性手套操作。
备注：产品信息可能会有优化升级。请以实际标签信息为准。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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