This product is a freeze-dried powder containing 6 protein mixtures with a molecular weight range of 14.4kD-97.4kD, and the content of each protein is about 20-30μg. After SDS-PAGE (polyacrylamide gel) electrophoresis, 6 bands with uniform distribution and similar density can be obtained by staining with Coomassie bright blue. It can be used to determine the molecular weight of proteins after SDS-PAGE electrophoresis.
Instructions for use:
1. After opening, dissolve in 400μL volume of 1× protein sample buffer (including β-mercaptoethanol) and heat in boiling water bath for 5 minutes. After cooling, it can be divided into small quantities according to needs, each tube is 20μL, stored at -20℃, and one tube is taken each time for use.
2. If it is used after long-term storage, it is best to preheat the small tube of boiling water bath after packaging for 3-5 minutes before use, and then apply the sample electrophoresis. 6 protein bands can be seen after dyeing with Coomasie bright blue G-250 (see the diagram below).
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